Istanbul Health and Technology University INFORMATION PACKAGE / COURSE CATALOGUE
| Pharmacy | |||||
| Bachelor | TR-NQF-HE: Level 6 | QF-EHEA: First Cycle | EQF-LLL: Level 6 | ||
Course General Introduction Information
| Course Code: | ECZ418 | ||||
| Course Name: | Pharmaceutical Toxicology Application | ||||
| Course Semester: | Fall | ||||
| Course Credits: |
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| Language of instruction: | TR-EN | ||||
| Course Requirement: | |||||
| Does the Course Require Work Experience?: | No | ||||
| Type of course: | Necessary | ||||
| Course Level: |
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| Mode of Delivery: | Face to face | ||||
| Course Coordinator : | Prof. Dr. BÜKET ALPERTUNGA | ||||
| Course Lecturer(s): |
Prof. Dr. BÜKET ALPERTUNGA |
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| Course Assistants: |
Course Purpose and Content
| Course Objectives: | Within the scope of this course, extraction and analysis of acidic and basic drugs, determination of volatile poisons, metallic poisons, DNA isolation and in vitro cytotoxicity testing will be done by using analytical methods. |
| Course Content: | Analysis methods in toxicology; Sample selection in systematic toxicological analysis; Isolation of poisons in systematic toxicological analysis; Determination of volatile substances in biological sample by microdiffusion technique; Spectrophotometric determination of methanol in biological fluids; Analysis of metallic poisons- Reinsch test; Determination of methemoglobin in blood; Acute toxicity tests (demonstration); In vitro cytotoxicity test -Trypan blue test; In vitro cytotoxicity test - MTT test; Genotoxicity test-Ames test (demonstration); DNA Damage analysis-Comet Analysis (demonstration) |
Learning Outcomes
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The students who have succeeded in this course;
1) Should know the basic analysis methods used in toxicology. 2) To be able to isolate poisons from biological fluids. 3) Should be able to know and apply the basis of systematic toxicological analysis. 4) Should be able to apply rapid tests used in the identification of poisons. 5) To be able to determine methemoglobin in blood. 6) Should be able to perform spectrophotometric quantitative analysis of methanol in biological fluids. 7) Should have knowledge about in vitro cytotoxicity tests and their applications. 8) Should have knowledge about genotoxicity tests and their applications. 9) Should have knowledge about DNA damage determination methods and their applications. 10) Should have knowledge about acute toxicity tests and their applications. |
Course Flow Plan
| Week | Subject | Related Preparation |
| 1) | Analysis methods in toxicology | -- |
| 2) | Sample selection in systematic toxicological analysis | -- |
| 3) | Isolation of poisons in systematic toxicological analysis | -- |
| 4) | Determination of volatile substances in biological sample by microdiffusion technique | -- |
| 5) | Extraction and identification of drugs causing poisoning in biological samples | -- |
| 6) | Spectrophotometric quantitative analysis of paracetamol in biological fluids | -- |
| 7) | Analysis of metallic poisons-Reinsch test | -- |
| 8) | Spectrophotometric quantitative analysis of methanol in biological fluids | -- |
| 9) | Determination of methemoglobin in blood | -- |
| 10) | Acute toxicity tests (demonstration) | -- |
| 11) | In vitro cytotoxicity test -Trypan blue test | -- |
| 12) | In vitro cytotoxicity test - MTT test | -- |
| 13) | Genotoxicity test-Ames test (demonstration) | -- |
| 14) | DNA Damage analysis-Comet Analysis (demonstration) | -- |
Sources
| Course Notes / Textbooks: | 1. Casarett and Doull’s Essentials of Toxicology, Claassen, C.D., Watkins J. B., , Lange Medical Books/McGraw-Hill, 2021. 2. Clarke's Analysis of Drugs and Poisons, Moffat AC, Osselton MD, Widdop B, Watts J, 4th Edition, Pharmaceutical Press, 2011. 3. Principles and Methods of Toxicology, Hayes AW, Taylor & Francis, 2007. |
| References: | 1. Farmasotik Toksikoloji, (Gerard J. Mulder, Lennart Dencker, Nobel Yayinevi, 2013. 2. Toksikolojinin Temelleri, Casarett & Doull’s, Ankara Nobel Tip Kitabevi, 2017. 3. Clarke's Analysis of Drugs and Poisons, Moffat AC, Osselton MD, Widdop B, Watts J, 4th Edition, Pharmaceutical Press, 2011. 4. Principles and Methods of Toxicology, Hayes AW, Taylor & Francis, 2007. |
Course - Learning Outcome Relationship
| No Effect | 1 Lowest | 2 Medium | 3 Highest |
| Program Outcomes | Level of Contribution | |
| 1) | Access, evaluate and apply up-to-date scientific knowledge in the field of pharmacy | 3 |
| 2) | It conveys information to patients, health personnel and society on all matters related to drugs. | 1 |
| 3) | Has the necessary knowledge about the therapeutic effects, drug forms, pharmacokinetic properties, stability and incompatibilities of drug active substances and can use this information. | 1 |
| 4) | Recognizes, controls and standardizes quality assurance issues and processes of all pharmaceutical-related products. | 2 |
| 5) | Has knowledge and equipment about the administration and use of the drug to the patient, advises on rational and safe drug use, and supports pharmacovigilance practices. | 1 |
| 6) | Has knowledge about the production and production technologies of natural and synthetic pharmaceutical raw materials. | 1 |
| 7) | Have knowledge about the concepts related to the functioning, maintenance and pathology of biological systems that form the basis of human health. | 2 |
| 8) | Has sufficient knowledge and application skills on research, development and production of pharmaceutical and cosmetic products. | 3 |
| 9) | Plans and implements all subjects within the scope of pharmacy services in the hospital. | 2 |
| 10) | Has knowledge about licensing conditions, data protection, patent and pharmacoeconomics in pharmaceuticals. | 1 |
| 11) | She/He is administratively and technically equipped in every field where the pharmacy profession is responsible by law, and produces solutions to problems in line with scientific data / evidence. | 1 |
| 12) | Combines basic and applied sciences with pharmacy profession practices and uses them in the field of pharmacy. | 3 |
| 13) | Has sufficient knowledge and application skills on physical, chemical, biological and toxicological analyzes of pharmaceutical, biotechnological and radiopharmaceutical products. | 5 |
| 14) | Acts in accordance with ethical and legal principles in the field of pharmacy. | 3 |
| 15) | Has the necessary competencies to work with colleagues and other health professionals in terms of communicative, managerial and social skills. | 4 |
| 16) | Provides consultancy on medicinal plants, herbal supplements and food supplements. | 1 |
| 17) | Knows pharmaceutical care practices, understands and evaluates drug interactions. | 1 |
| 18) | Knows the risks associated with the use of drugs and other chemical substances and laboratory practice. | 5 |
Learning Activity and Teaching Methods
| Anlatım | |
| Labs | |
| Okuma | |
| Soru cevap/ Tartışma |
Measurement and Evaluation Methods and Criteria
| Yazılı Sınav (Açık uçlu sorular, çoktan seçmeli, doğru yanlış, eşleştirme, boşluk doldurma, sıralama) | |
| Uygulama | |
| Gözlem |
Assessment & Grading
| Semester Requirements | Number of Activities | Level of Contribution |
| Midterms | 1 | % 40 |
| Final | 1 | % 60 |
| total | % 100 | |
| PERCENTAGE OF SEMESTER WORK | % 40 | |
| PERCENTAGE OF FINAL WORK | % 60 | |
| total | % 100 | |
İş Yükü ve AKTS Kredisi Hesaplaması
| Activities | Number of Activities | Aktiviteye Hazırlık | Aktivitede Harçanan Süre | Aktivite Gereksinimi İçin Süre | Workload | ||
| Laboratory | 14 | 3 | 42 | ||||
| Study Hours Out of Class | 14 | 1 | 14 | ||||
| Midterms | 1 | 1 | 1 | ||||
| Final | 1 | 1 | 1 | ||||
| Total Workload | 58 | ||||||