Molecular Biology and Genetics Preview
Bachelor	TR-NQF-HE: Level 6	QF-EHEA: First Cycle	EQF-LLL: Level 6

Course General Introduction Information

Course Code:

MBG206

Course Name:

Microbiology Laboratory

Course Semester:

Spring

Course Credits:

ECTS

Language of instruction:

Course Requirement:

Does the Course Require Work Experience?:

Type of course:

Necessary

Course Level:

Bachelor

TR-NQF-HE:6. Master`s Degree

QF-EHEA:First Cycle

EQF-LLL:6. Master`s Degree

Mode of Delivery:

Face to face

Course Coordinator :

Dr.Öğr.Üyesi MERVE BİLGİN

Course Lecturer(s):

Dr. Merve Bilgin

Course Assistants:

Course Purpose and Content

Course Objectives:

The purpose of this course is to acquire professional skills in microbiology laboratory by showing the basic working principles and uses of the tools and devices in the microbiology laboratory, as well as the microbiological control of pharmaceutical products, air and water, diagnosis of isolated microorganisms, antibiotic susceptibility and antibiotic combination assays.

Course Content:

1. Why a Microbiology laboratory? Laboratory rules, introduction of tools and devices.
2. Preparing a slide preparation from the mouth and saliva, and examining the microscope with dry system objectives.
3. Examination of microorganisms after simple staining method with immersion objective of the microscope.
4. Gram staining method.
5. Culture Media and culture methods
6. Examination of blood smear preparation with Giemsa staining method.
7. Antibiotic susceptibility testing methods: Investigation susceptibility of bacteria to antibiotics by disc diffusion method.
8. Antibiotic combination tests against bacteria.
9. Examination of fungi by staining methods. / Hand hygiene control.
10. Examination of parasite preparations.
11. Microbiological examination of air and water.
12. Blood group determination
13. Biofilm formation test (demo) and some biofilm applications.
14. Microbiological control of sterile and non-sterile pharmaceutical products.

Learning Outcomes

The students who have succeeded in this course;
1) Molecular biologists who are open to scientific developments in pharmacy and related fields, combine their knowledge and creativity, and contribute to society and industry in education, research, and practice should be trained.

Course Flow Plan

Week	Subject	Related Preparation
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Sources

Course Notes / Textbooks:

1. Warren Levinson (Çeviri editörleri: Burçin Şener, Berrin Esen), Tıbbi Mikrobiyoloji ve İmmünoloji, Güneş Tıp Kitabevleri, 2017
2. Patrick R. Murray (Çeviri editörleri: A. Dürdal US, Ahmet BAŞUSTAOĞLU), Temel Tıbbi Mikrobiyoloji, Elsevier, 2018
3. Ahmet Başustaoğlu, Dürdal Us, Antisepsi, Dezenfeksiyon ve Sterilizasyon, Hipokrat Kitabevi, 2019
Yardımcı Kaynaklar
1. Çotuk, A. Genel Mikrobiyoloji Laboratuvar Yöntemleri. İstanbul Üniversitesi Fen Fakültesi Biyoloji Bölümü. Nobel Tıp Kitabevleri, 2003
2. Patricia Tille, Bailey & Scott's Diagnostic Microbiology, 15th Edition, Elsevier, 2021

References:

1. Warren Levinson, Review of Medical Microbiology and Immunology, Lange, 15th edition, 2016
SUPPLEMENTARY SOURCES
1. Patricia Tille, Bailey & Scott's Diagnostic Microbiology, 15th Edition, Elsevier, 2021

Course - Learning Outcome Relationship

No Effect	1 Lowest	2 Medium	3 Highest

	Program Outcomes	Level of Contribution
1)	Having knowledge and understanding of Molecular Biology and Genetics subjects, established on competencies gained in previous education and supported by using course books containing latest information, application tools and other scientific literature.
2)	Students can integrate knowledge and skills from molecular biology and genetics courses and can acquire further knowledge according to their own interests.
3)	Students acquire practical skills in fundamental molecular biology and genetics techniques.
4)	Ability of proposing solutions in unexpected, complicated situations on applications of Molecular Biology and Genetics by claiming responsibility individually or as a part of a team.
5)	Competency in planning academic studies on Molecular Biology and Genetics and carrying out these studies individually or collectively.
6)	Students can develop ability to analyse and interpret experimental data obtained in a laboratory setting statistically.
7)	Sufficient foreign language knowledge for communication between colleagues and following literature on Molecular Biology and Genetics.
8)	Students can use computational technologies to analyse scientific data and for information retrieval.
9)	Being aware of the necessity of lifelong education, reaching information, following the advances in science and technology and constant struggle of renewing oneself.
10)	Evaluating natural and social events with an environmental point of view and ability of informing and leading the public opinion
11)	Students acquire professional knowledge and skills to fulfil requirements of their future employers.
12)	Having proper social, ethical and scientific values and the will to protect these values on studies about collection, evaluation, contemplation, publication and application of data regarding Molecular Biology and Genetics.
13)	Students can understand and evaluate advantages and limitations of technological platforms in life sciences including genomics, genetic engineering and biotechnology.
14)	Students would have consciousness on subjects such as the quality management, worker welfare and safety.

Learning Activity and Teaching Methods

Labs

Measurement and Evaluation Methods and Criteria

Yazılı Sınav (Açık uçlu sorular, çoktan seçmeli, doğru yanlış, eşleştirme, boşluk doldurma, sıralama)
Uygulama
Raporlama

Assessment & Grading

Semester Requirements	Number of Activities	Level of Contribution
Midterms	1	% 40
Final	1	% 60
total		% 100
PERCENTAGE OF SEMESTER WORK		% 40
PERCENTAGE OF FINAL WORK		% 60
total		% 100

İş Yükü ve AKTS Kredisi Hesaplaması

Activities	Number of Activities	Aktiviteye Hazırlık	Aktivitede Harçanan Süre	Aktivite Gereksinimi İçin Süre	Workload
Course Hours	14	3			42
Study Hours Out of Class	14	4			56
Midterms	1	1			1
Final	1	1			1
Total Workload					100