Istanbul Health and Technology University INFORMATION PACKAGE / COURSE CATALOGUE
| Molecular Biology and Genetics | |||||
| Bachelor | TR-NQF-HE: Level 6 | QF-EHEA: First Cycle | EQF-LLL: Level 6 | ||
Course General Introduction Information
| Course Code: | MBG308 | ||||
| Course Name: | Recombinant DNA Technologies and Gene Expression Analysis | ||||
| Course Semester: | Spring | ||||
| Course Credits: |
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| Language of instruction: | TR | ||||
| Course Requirement: | |||||
| Does the Course Require Work Experience?: | No | ||||
| Type of course: | Necessary | ||||
| Course Level: |
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| Mode of Delivery: | Face to face | ||||
| Course Coordinator : | Dr.Öğr.Üyesi EBUZER KALYONCU | ||||
| Course Lecturer(s): |
Dr.Öğr.Üyesi EBUZER KALYONCU |
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| Course Assistants: |
Course Purpose and Content
| Course Objectives: | The aim of this course is to enable students to learn gene cloning techniques and non-cloning approaches, especially the polymerase chain reaction (PCR) method, which form the basis of genetic and molecular biology studies. It also aims to provide them with information about the methods used to sequence genomes and analyze the resulting sequences. In the last part of the course, it is aimed to provide students with information about the research methods used in DNA analysis and gene expression analysis. |
| Course Content: | Part I: Basic Principles of Gene Cloning and DNA Analysis Part II. Applications in Research and Gene Cloning and DNA Analysis |
Learning Outcomes
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The students who have succeeded in this course;
1) Students who successfully complete this course: Gene Cloning Techniques: They will understand the basic principles and applications of gene cloning techniques and will be able to apply these techniques in the laboratory environment. Non-cloning Approaches: By learning non-cloning approaches such as polymerase chain reaction (PCR), they will be able to use these techniques effectively in genetic analyses. Genome Sequencing and Analysis: They will understand genome sequencing technologies and learn how to analyze the obtained sequences using bioinformatics methods. DNA and Gene Expression Analysis: By understanding the methods used in DNA analysis and gene expression analysis in research, they will be able to apply these techniques in their experimental studies. Problem Solving and Research: They will be able to select and apply appropriate techniques and carry out research projects to solve problems encountered in the field of genetics and molecular biology. Data Interpretation: They will be able to interpret the data obtained as a result of genome sequencing and gene expression analyzes and present these data in the form of scientific reports. Current Technologies: They will be able to follow current technologies used in recombinant DNA technologies and gene expression analysis and adapt to innovations in this field. |
Course Flow Plan
| Week | Subject | Related Preparation |
| 1) | Gene Cloning and DNA Analysis | |
| 2) | Vectors for Gene Cloning | |
| 3) | Purification of DNA from Living Cells | |
| 4) | Manipulation of Purified DNA | |
| 5) | Entry of DNA into Living Cells | |
| 6) | Cloning Vectors for E.coli | |
| 7) | Cloning Vectors for Eukaryotes | |
| 8) | Midterm exam | |
| 9) | How to Obtain a Clone Containing a Specific Gene? | |
| 10) | Polymerase Chain Reaction | |
| 11) | Examining the Location and Structure of the Gene | |
| 12) | Examining Gene Expression | |
| 13) | Examining Gene Function | |
| 14) | Studying Genomes |
Sources
| Course Notes / Textbooks: | |
| References: | GEN KLONLAMA ve DNA ANALİZİ: Giriş / Gene Cloning and DNA Analysis: An Introduction |
Course - Learning Outcome Relationship
| No Effect | 1 Lowest | 2 Medium | 3 Highest |
| Program Outcomes | Level of Contribution | |
| 1) | Having knowledge and understanding of Molecular Biology and Genetics subjects, established on competencies gained in previous education and supported by using course books containing latest information, application tools and other scientific literature. | 2 |
| 2) | Students can integrate knowledge and skills from molecular biology and genetics courses and can acquire further knowledge according to their own interests. | 3 |
| 3) | Students acquire practical skills in fundamental molecular biology and genetics techniques. | 3 |
| 4) | Ability of proposing solutions in unexpected, complicated situations on applications of Molecular Biology and Genetics by claiming responsibility individually or as a part of a team. | 1 |
| 5) | Competency in planning academic studies on Molecular Biology and Genetics and carrying out these studies individually or collectively. | 1 |
| 6) | Students can develop ability to analyse and interpret experimental data obtained in a laboratory setting statistically. | 3 |
| 7) | Sufficient foreign language knowledge for communication between colleagues and following literature on Molecular Biology and Genetics. | 3 |
| 8) | Students can use computational technologies to analyse scientific data and for information retrieval. | 3 |
| 9) | Being aware of the necessity of lifelong education, reaching information, following the advances in science and technology and constant struggle of renewing oneself. | 2 |
| 10) | Evaluating natural and social events with an environmental point of view and ability of informing and leading the public opinion | 1 |
| 11) | Students acquire professional knowledge and skills to fulfil requirements of their future employers. | 3 |
| 12) | Having proper social, ethical and scientific values and the will to protect these values on studies about collection, evaluation, contemplation, publication and application of data regarding Molecular Biology and Genetics. | 1 |
| 13) | Students can understand and evaluate advantages and limitations of technological platforms in life sciences including genomics, genetic engineering and biotechnology. | 3 |
| 14) | Students would have consciousness on subjects such as the quality management, worker welfare and safety. | 1 |
Learning Activity and Teaching Methods
| Course | |
| Okuma | |
| Homework | |
| Problem Çözme | |
| Soru cevap/ Tartışma |
Measurement and Evaluation Methods and Criteria
| Yazılı Sınav (Açık uçlu sorular, çoktan seçmeli, doğru yanlış, eşleştirme, boşluk doldurma, sıralama) | |
| Homework |
Assessment & Grading
| Semester Requirements | Number of Activities | Level of Contribution |
| Midterms | 1 | % 40 |
| Final | 1 | % 60 |
| total | % 100 | |
| PERCENTAGE OF SEMESTER WORK | % 40 | |
| PERCENTAGE OF FINAL WORK | % 60 | |
| total | % 100 | |
İş Yükü ve AKTS Kredisi Hesaplaması
| Activities | Number of Activities | Aktiviteye Hazırlık | Aktivitede Harçanan Süre | Aktivite Gereksinimi İçin Süre | Workload | ||
| Course Hours | 14 | 3 | 42 | ||||
| Midterms | 1 | 27 | 27 | ||||
| Final | 1 | 36 | 36 | ||||
| Total Workload | 105 | ||||||