Istanbul Health and Technology University INFORMATION PACKAGE / COURSE CATALOGUE
| Molecular Biology and Genetics | |||||
| Bachelor | TR-NQF-HE: Level 6 | QF-EHEA: First Cycle | EQF-LLL: Level 6 | ||
Course General Introduction Information
| Course Code: | MBG301 | ||||
| Course Name: | Gene Expression Regulation | ||||
| Course Semester: | Fall | ||||
| Course Credits: |
|
||||
| Language of instruction: | |||||
| Course Requirement: | |||||
| Does the Course Require Work Experience?: | No | ||||
| Type of course: | Necessary | ||||
| Course Level: |
|
||||
| Mode of Delivery: | Face to face | ||||
| Course Coordinator : | Dr.Öğr.Üyesi SONER AKSU | ||||
| Course Lecturer(s): | Soner Aksu, PhD | ||||
| Course Assistants: |
Course Purpose and Content
| Course Objectives: | The aim of this course is to develop knowledge and understanding about the mechanisms by which eukaryotes regulate gene expression through transcription, chromatin, mRNA stability and control of gene expression. |
| Course Content: | 1. Introduction to gene expression, genetic code and its properties, basic functioning of the genetic code, decoding of the code 2. Wooble hypothesis, degenerate cipher, RNA polymerase 3. Prokaryotic and eukaryotic gene transcription, elements and motifs associated with gene expression regulation 4. mRNA processing: 5‘-end head and 3’-end polyA tail structures 5. mRNA processing: splicing and splice melanisms, UTR sequences 6. mRNA transport and regulation, mRNA stabilisation mechanisms 7. Introduction to the regulation of prokaryotic gene expression 8. lac operon mechanism and effect of mutations, CAp-cAMP regulation model 9. Regulation of prokaryotic gene expression by trp operon and RNA secondary structures and intermediate operon 10. Introduction to eukaryotic gene expression 11. The world of RNA: RNA molecules affecting eukaryotic gene expression 12. Histone modifications and nucleosome structure changes in gene expression 13. Cis-acting elements and trans-acting factors in the regulation of eukaryotic gene expression 14. alternative splicing of mRNA, RNA silencing |
Learning Outcomes
|
The students who have succeeded in this course;
1) Basic concepts in the regulation of gene expression, 2) Basic mechanisms of prokaryotic gene expression and regulation of these mechanisms, 3) Transcriptional, post-transcriptional regulation of eukaryotic gene expression 4) mRNA structure and mRNA processing 5) Other RNA molecules involved in the regulation of gene expression, 6) Cis-acting elements, conserved motifs and trans-acting factors on gene expression 7) Histone modifications, nucleosome structure changes and RNA polymerases 8) Alternative splicing and RNA silencing mechanism 9) Programmed DNA rearrangements and gene expression regulation |
Course Flow Plan
| Week | Subject | Related Preparation |
| 1) | 1. Introduction to gene expression, genetic code and its properties, basic functioning of the genetic code, decoding of the code 2. Wooble hypothesis, degenerate cipher, RNA polymerase 3. Prokaryotic and eukaryotic gene transcription, elements and motifs associated with gene expression regulation 4. mRNA processing: 5‘-end head and 3’-end polyA tail structures 5. mRNA processing: splicing and splice melanisms, UTR sequences 6. mRNA transport and regulation, mRNA stabilisation mechanisms 7. Introduction to the regulation of prokaryotic gene expression 8. lac operon mechanism and effect of mutations, CAp-cAMP regulation model 9. Regulation of prokaryotic gene expression by trp operon and RNA secondary structures and intermediate operon 10. Introduction to eukaryotic gene expression 11. The world of RNA: RNA molecules affecting eukaryotic gene expression 12. Histone modifications and nucleosome structure changes in gene expression 13. Cis-acting elements and trans-acting factors in the regulation of eukaryotic gene expression 14. alternative splicing of mRNA, RNA silencing | Lecture notes and presentations |
Sources
| Course Notes / Textbooks: | William Klug, Michael Cummings, Charlotte Spencer, Michael Palladino – Genetik Kavramlar, 11. Baskı David Latchman – Gene Regulation: A eukaryotic perspective – 3rd Edition Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Matthew P. Scott, Anthony Bretschler, Hidde Ploegh, Paul Matsudaira – Moleküler Hücre Biyolojisi, 6. Baskı |
| References: | William Klug, Michael Cummings, Charlotte Spencer, Michael Palladino – Concepts of Genetics, 11st Edition David Latchman – Gene Regulation: A eukaryotic perspective – 3rd Edition Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Matthew P. Scott, Anthony Bretschler, Hidde Ploegh, Paul Matsudaira – Molecular Cell Biology, 6th Edition |
Course - Learning Outcome Relationship
| No Effect | 1 Lowest | 2 Medium | 3 Highest |
| Program Outcomes | Level of Contribution | |
| 1) | Having knowledge and understanding of Molecular Biology and Genetics subjects, established on competencies gained in previous education and supported by using course books containing latest information, application tools and other scientific literature. | 2 |
| 2) | Students can integrate knowledge and skills from molecular biology and genetics courses and can acquire further knowledge according to their own interests. | 1 |
| 3) | Students acquire practical skills in fundamental molecular biology and genetics techniques. | |
| 4) | Ability of proposing solutions in unexpected, complicated situations on applications of Molecular Biology and Genetics by claiming responsibility individually or as a part of a team. | 2 |
| 5) | Competency in planning academic studies on Molecular Biology and Genetics and carrying out these studies individually or collectively. | 2 |
| 6) | Students can develop ability to analyse and interpret experimental data obtained in a laboratory setting statistically. | 1 |
| 7) | Sufficient foreign language knowledge for communication between colleagues and following literature on Molecular Biology and Genetics. | |
| 8) | Students can use computational technologies to analyse scientific data and for information retrieval. | 1 |
| 9) | Being aware of the necessity of lifelong education, reaching information, following the advances in science and technology and constant struggle of renewing oneself. | 3 |
| 10) | Evaluating natural and social events with an environmental point of view and ability of informing and leading the public opinion | 2 |
| 11) | Students acquire professional knowledge and skills to fulfil requirements of their future employers. | 3 |
| 12) | Having proper social, ethical and scientific values and the will to protect these values on studies about collection, evaluation, contemplation, publication and application of data regarding Molecular Biology and Genetics. | 2 |
| 13) | Students can understand and evaluate advantages and limitations of technological platforms in life sciences including genomics, genetic engineering and biotechnology. | 3 |
| 14) | Students would have consciousness on subjects such as the quality management, worker welfare and safety. |
Learning Activity and Teaching Methods
| Anlatım | |
| Bireysel çalışma ve ödevi | |
| Course | |
| Soru cevap/ Tartışma | |
| Örnek olay çalışması |
Measurement and Evaluation Methods and Criteria
| Yazılı Sınav (Açık uçlu sorular, çoktan seçmeli, doğru yanlış, eşleştirme, boşluk doldurma, sıralama) | |
| Homework | |
| Sunum |
Assessment & Grading
| Semester Requirements | Number of Activities | Level of Contribution |
| Homework Assignments | 4 | % 30 |
| Midterms | 1 | % 20 |
| Final | 1 | % 50 |
| total | % 100 | |
| PERCENTAGE OF SEMESTER WORK | % 50 | |
| PERCENTAGE OF FINAL WORK | % 50 | |
| total | % 100 | |
İş Yükü ve AKTS Kredisi Hesaplaması
| Activities | Number of Activities | Aktiviteye Hazırlık | Aktivitede Harçanan Süre | Aktivite Gereksinimi İçin Süre | Workload | ||
| Course Hours | 14 | 3 | 42 | ||||
| Study Hours Out of Class | 7 | 10 | 70 | ||||
| Homework Assignments | 5 | 5 | 25 | ||||
| Midterms | 1 | 1.5 | 1.5 | ||||
| Final | 1 | 1.5 | 1.5 | ||||
| Total Workload | 140 | ||||||