Istanbul Health and Technology University INFORMATION PACKAGE / COURSE CATALOGUE
| Molecular Biology and Genetics | |||||
| Bachelor | TR-NQF-HE: Level 6 | QF-EHEA: First Cycle | EQF-LLL: Level 6 | ||
Course General Introduction Information
| Course Code: | MBG403 | ||||
| Course Name: | Moleküler Klonlama Teknikleri Lab | ||||
| Course Semester: | Fall | ||||
| Course Credits: |
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| Language of instruction: | TR | ||||
| Course Requirement: | |||||
| Does the Course Require Work Experience?: | No | ||||
| Type of course: | Necessary | ||||
| Course Level: |
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| Mode of Delivery: | Face to face | ||||
| Course Coordinator : | Dr.Öğr.Üyesi EBUZER KALYONCU | ||||
| Course Lecturer(s): | |||||
| Course Assistants: |
Course Purpose and Content
| Course Objectives: | Molecular Cloning Laboratory Course Objectives Introduction to Synthetic Biology: Understand the fundamental concepts of synthetic biology and its multidisciplinary nature, focusing on its potential to address global challenges in drug discovery, energy production, and beyond. Practical Application of Synthetic Biology: Learn cutting-edge methods that simplify biological engineering, enabling students to apply these techniques in a laboratory setting. Lab Manual Utilization: Gain proficiency in using a comprehensive synthetic biology lab manual, which compiles essential methods and protocols for conducting experiments in synthetic biology. Project-Based Learning: Develop and execute student-driven projects that align with the principles of synthetic biology, fostering creativity and innovation in problem-solving. Preparation for iGEM Competition: Equip students with the skills and knowledge necessary to participate in the international Genetically Engineered Machine (iGEM) competition, including understanding and utilizing BioBricks™. Cross-Disciplinary Collaboration: Encourage collaboration among students from diverse scientific backgrounds, including biology, chemistry, engineering, and even high school students, to promote a holistic understanding of synthetic biology. Experimentation and Data Analysis: Conduct experiments using synthetic biology protocols, analyze the results, and draw meaningful conclusions to further scientific understanding. Ethical and Social Implications: Explore the ethical, legal, and social implications of synthetic biology, fostering a responsible approach to scientific research and its applications. Research Skills Development: Enhance research skills through hands-on laboratory experience, including experimental design, data collection, and interpretation. Innovation and Creativity: Inspire students to think creatively and innovatively, utilizing synthetic biology techniques to develop novel solutions to real-world problems. |
| Course Content: | 1- Introduction and Fundamentals -Introduction to synthetic biology -Course objectives and outcomes -Importance and applications of synthetic biology -Overview of iGEM competition and BioBricks™ 2-Lab Safety and Basic Techniques -Lab safety protocols -Basic laboratory techniques (pipetting, agar plates, bacterial culture) -Introduction to Escherichia coli (E. coli) as a model organism 3- Genes, Chromoproteins and Antisense RNAs 4- Lab section 1. Preparation of chemical solutions and agar plates 5- Lab section 2. Coloring bacteria by adding a promoter to a chromoprotein gene 6-Lab section 3: Rational engineering of chromoprotein expression level 7- Lab section 4. Other experiments |
Learning Outcomes
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The students who have succeeded in this course;
1) At the end of the lab course, students should be able to: • understand basic principles in synthetic biology; • perform experimental protocols central to synthetic biology; • use the scientific method to generate hypotheses, design experiments to test them and analyze the results; • document and present clearly their methods, results and conclusions |
Course Flow Plan
| Week | Subject | Related Preparation |
| 1) | Preparation of solutions and agar plates | |
| 2) | Overnight cultures with antibiotics, and glycerol stocks | |
| 3) | BioBrick™ 3A assembly and gel analysis | |
| 4) | Agarose gel electrophoresis | |
| 5) | Preparation of competent E. coli cells using CaCl2 | |
| 6) | Transformation of CaCl2-competent E. coli cells | |
| 7) | Bacterial re-streaking techniques | |
| 8) | Lysis of E. coli cells with lysozyme | |
| 9) | Polymerase chain reaction (PCR) | |
| 10) | Inverse PCR mutagenesis | |
| 11) | Colony PCR | |
| 12) | Gibson assembly |
Sources
| Course Notes / Textbooks: | |
| References: | Sentetik Biyoloji - Laboratuvar Kılavuzu MBG 403- Moleküler klonlama laboratuvar kılavuzu Synthetic Biology - A Lab Manual MBG 403- Molecular cloning laboratory guide |
Course - Learning Outcome Relationship
| No Effect | 1 Lowest | 2 Medium | 3 Highest |
| Program Outcomes | Level of Contribution | |
| 1) | Having knowledge and understanding of Molecular Biology and Genetics subjects, established on competencies gained in previous education and supported by using course books containing latest information, application tools and other scientific literature. | 3 |
| 2) | Students can integrate knowledge and skills from molecular biology and genetics courses and can acquire further knowledge according to their own interests. | 3 |
| 3) | Students acquire practical skills in fundamental molecular biology and genetics techniques. | 3 |
| 4) | Ability of proposing solutions in unexpected, complicated situations on applications of Molecular Biology and Genetics by claiming responsibility individually or as a part of a team. | 3 |
| 5) | Competency in planning academic studies on Molecular Biology and Genetics and carrying out these studies individually or collectively. | 3 |
| 6) | Students can develop ability to analyse and interpret experimental data obtained in a laboratory setting statistically. | 3 |
| 7) | Sufficient foreign language knowledge for communication between colleagues and following literature on Molecular Biology and Genetics. | 3 |
| 8) | Students can use computational technologies to analyse scientific data and for information retrieval. | 3 |
| 9) | Being aware of the necessity of lifelong education, reaching information, following the advances in science and technology and constant struggle of renewing oneself. | 3 |
| 10) | Evaluating natural and social events with an environmental point of view and ability of informing and leading the public opinion | 1 |
| 11) | Students acquire professional knowledge and skills to fulfil requirements of their future employers. | 3 |
| 12) | Having proper social, ethical and scientific values and the will to protect these values on studies about collection, evaluation, contemplation, publication and application of data regarding Molecular Biology and Genetics. | 1 |
| 13) | Students can understand and evaluate advantages and limitations of technological platforms in life sciences including genomics, genetic engineering and biotechnology. | 3 |
| 14) | Students would have consciousness on subjects such as the quality management, worker welfare and safety. | 2 |
Learning Activity and Teaching Methods
| Grup çalışması ve ödevi | |
| Labs | |
| Problem Çözme |
Measurement and Evaluation Methods and Criteria
| Uygulama | |
| Grup Projesi | |
| Sunum | |
| Raporlama |
Assessment & Grading
| Semester Requirements | Number of Activities | Level of Contribution |
| Laboratory | 1 | % 30 |
| Presentation | 1 | % 40 |
| Final | 1 | % 30 |
| total | % 100 | |
| PERCENTAGE OF SEMESTER WORK | % 70 | |
| PERCENTAGE OF FINAL WORK | % 30 | |
| total | % 100 | |
İş Yükü ve AKTS Kredisi Hesaplaması
| Activities | Number of Activities | Aktiviteye Hazırlık | Aktivitede Harçanan Süre | Aktivite Gereksinimi İçin Süre | Workload | ||
| Course Hours | 12 | 4 | 48 | ||||
| Presentations / Seminar | 1 | 30 | 30 | ||||
| Paper Submission | 1 | 30 | 30 | ||||
| Final | 1 | 10 | 10 | ||||
| Total Workload | 118 | ||||||