MBG307 Biochemistry LabInstitutional InformationDegree Programs Molecular Biology and GeneticsInformation For StudentsDiploma SupplementErasmus Policy StatementNational Qualifications
Molecular Biology and Genetics

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Bachelor TR-NQF-HE: Level 6 QF-EHEA: First Cycle EQF-LLL: Level 6

Course General Introduction Information

Course Code: MBG307
Course Name: Biochemistry Lab
Course Semester: Fall
Course Credits:
ECTS
4
Language of instruction:
Course Requirement:
Does the Course Require Work Experience?: No
Type of course: Necessary
Course Level:
Bachelor TR-NQF-HE:6. Master`s Degree QF-EHEA:First Cycle EQF-LLL:6. Master`s Degree
Mode of Delivery:
Course Coordinator : Ar.Gör. BAHAR İNCE
Course Lecturer(s): Dr. Soner Aksu
Course Assistants:

Course Purpose and Content

Course Objectives: Reinforcing the basic knowledge learned in biochemistry course with laboratory applications
Course Content: Application areas of calorimetric, potentiometric, electrophoretic and chromatographic methods

Learning Outcomes

The students who have succeeded in this course;
1) Students should be able to gain the ability to apply biochemical research methods.
2) Must be able to prepare buffer solution.
3) Must be able to calculate the amount of protein.
4) Must be able to perform column chromatography.
5) Must be able to perform SDS gel electrophoresis
6) Must be able to make basic enzyme activity measurements.
7) Must be able to perform DNA isolation and analysis.

Course Flow Plan

Week Subject Related Preparation
1) Introduction and Preparation of Buffer Solutions Pre-prepared presentations and laboratory sheets
2) Titration of Amino Acids Pre-prepared presentations and laboratory sheets
3) Colorimetric Methods for Determination of Amino Acids and Proteins (Biuret and Lowry) Pre-prepared presentations and laboratory sheets
4) Introduction to Chromatographic Techniques I (Paper Chromatography) Pre-prepared presentations and laboratory sheets
5) Introduction to Chromatographic Techniques II (Column Chromatography) Pre-prepared presentations and laboratory sheets
6) Electrophoresis of Amino Acids and Proteins (SDS PAGE) Pre-prepared presentations and laboratory sheets
7) Preparation of Mitochondria from Rat Liver Pre-prepared presentations and laboratory sheets
8) Midterm
9) Photosynthesis (Chlorophyll Isolation, Paper Chromatography of Pigments and PS-II Activity Measurements) Pre-prepared presentations and laboratory sheets
10) Enzyme Kinetics Catalase (substrate / enzyme concentration / pH / temperature) Pre-prepared presentations and laboratory sheets
11) DNA Preparation (Plant Genomic DNA isolation with CTAB method) Pre-prepared presentations and laboratory sheets
12) Properties of DNA (Tm-curve, Agarose Gel Electrophoresis and qPCR) Pre-prepared presentations and laboratory sheets
13) Repeat application Pre-prepared presentations and laboratory sheets
14) Repeat application Pre-prepared presentations and laboratory sheets
15) Repeat application Pre-prepared presentations and laboratory sheets
16) Final exam
17) Final Exam

Sources

Course Notes / Textbooks: Önceden hazırlanmış sunumlar ve laboratuvar föyü
References: Pre-prepared presentations and laboratory sheets

Course - Learning Outcome Relationship

No Effect 1 Lowest 2 Medium 3 Highest
       
Program Outcomes Level of Contribution
1) Having knowledge and understanding of Molecular Biology and Genetics subjects, established on competencies gained in previous education and supported by using course books containing latest information, application tools and other scientific literature. 3
2) Students can integrate knowledge and skills from molecular biology and genetics courses and can acquire further knowledge according to their own interests. 2
3) Students acquire practical skills in fundamental molecular biology and genetics techniques. 3
4) Ability of proposing solutions in unexpected, complicated situations on applications of Molecular Biology and Genetics by claiming responsibility individually or as a part of a team. 3
5) Competency in planning academic studies on Molecular Biology and Genetics and carrying out these studies individually or collectively. 2
6) Students can develop ability to analyse and interpret experimental data obtained in a laboratory setting statistically. 2
7) Sufficient foreign language knowledge for communication between colleagues and following literature on Molecular Biology and Genetics. 3
8) Students can use computational technologies to analyse scientific data and for information retrieval. 2
9) Being aware of the necessity of lifelong education, reaching information, following the advances in science and technology and constant struggle of renewing oneself. 3
10) Evaluating natural and social events with an environmental point of view and ability of informing and leading the public opinion 2
11) Students acquire professional knowledge and skills to fulfil requirements of their future employers. 3
12) Having proper social, ethical and scientific values and the will to protect these values on studies about collection, evaluation, contemplation, publication and application of data regarding Molecular Biology and Genetics. 1
13) Students can understand and evaluate advantages and limitations of technological platforms in life sciences including genomics, genetic engineering and biotechnology. 2
14) Students would have consciousness on subjects such as the quality management, worker welfare and safety. 2

Learning Activity and Teaching Methods

Alan Çalışması
Grup çalışması ve ödevi
Labs
Rapor Yazma
Staj/Yerinde Uygulama

Measurement and Evaluation Methods and Criteria

Uygulama
Raporlama

Assessment & Grading

Semester Requirements Number of Activities Level of Contribution
Laboratory 14 % 40
Midterms 1 % 30
Final 1 % 30
total % 100
PERCENTAGE OF SEMESTER WORK % 70
PERCENTAGE OF FINAL WORK % 30
total % 100

İş Yükü ve AKTS Kredisi Hesaplaması

Activities Number of Activities Aktiviteye Hazırlık Aktivitede Harçanan Süre Aktivite Gereksinimi İçin Süre Workload
Application 17 2 34
Study Hours Out of Class 14 2 28
Midterms 1 24 24
Final 1 24 24
Total Workload 110