MBG203 Genetics LaboratoryInstitutional InformationDegree Programs Molecular Biology and GeneticsInformation For StudentsDiploma SupplementErasmus Policy StatementNational Qualifications
Molecular Biology and Genetics

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Bachelor TR-NQF-HE: Level 6 QF-EHEA: First Cycle EQF-LLL: Level 6

Course General Introduction Information

Course Code: MBG203
Course Name: Genetics Laboratory
Course Semester: Fall
Course Credits:
ECTS
6
Language of instruction:
Course Requirement:
Does the Course Require Work Experience?: No
Type of course: Necessary
Course Level:
Bachelor TR-NQF-HE:6. Master`s Degree QF-EHEA:First Cycle EQF-LLL:6. Master`s Degree
Mode of Delivery: Face to face
Course Coordinator : Ar.Gör. BAHAR İNCE
Course Lecturer(s): Lecturer Fatma Kübra Ata
Course Assistants:

Course Purpose and Content

Course Objectives: To learn basic information about the use of Drosophila melanogaster and Caenorhabditis elegans as model organisms in genetic studies. Crossbreeding, progeny and character analysis of D. melanogaster. Culturing, synchronization and enumeration of C.elegans. Examination of concepts and problems related to monohybrid and dihybrid crosses. Karyotype analysis, blood group determination, Barr body and giant chromosomes will be learned in this course.
Course Content: Life Cycle, Mitosis and Meiosis, Crossover

Learning Outcomes

The students who have succeeded in this course;
1) Providing F1 and F2 offspring
2) Understanding the genetic basis of blood groups, identifying Barr bodies and giant chromosomes
3) Being able to do karyotyping
4) Being able to do karyotyping
5) To grow Drosophila melanogaster and Caenorhabditis elegans as model organisms under laboratory conditions
6) To be able to cross any type of fruit flies under controlled conditions

Course Flow Plan

Week Subject Related Preparation
1) Drosophila melanogaster Life Cycle, Examination under Dissection Microscope, Differentiation of Male and Female Individuals, stunning, medium preparation Pre-prepared presentation and test sheet
2) Virgin female extraction, problems with Mitosis and Meiosis, (Virgin female extraction continues throughout the week) Pre-prepared presentation and test sheet
3) Crossover, Monohybrid crossover problems Pre-prepared presentation and test sheet
4) Separation of parents, Dihybrid cross problems Pre-prepared presentation and test sheet
5) F1 Male and female counting, obtaining virgin female from F1 (continues throughout the week) Pre-prepared presentation and test sheet
6) F2 crossover, chi-square and equivalence problems Pre-prepared presentation and test sheet
7) Separation of parents, chi-square and equivalence problems Pre-prepared presentation and test sheet
8) Midterm
9) Counting of F2 offspring, Karyotype analysis Pre-prepared presentation and test sheet
10) Blood groups and analysis, related problems Pre-prepared presentation and test sheet
11) Examination of the Barr body, related problems Pre-prepared presentation and test sheet
12) Examination of giant chromosomes Pre-prepared presentation and test sheet
13) Caenorhabditis elegans life cycle, Stereomicroscope examination, differentiation of eggs, larvae and adults, passage Pre-prepared presentation and test sheet
14) Synchronizing Caenorhabditis elegans populations, egg retrieval Pre-prepared presentation and test sheet
15) Caenorhabditis elegans individual census Pre-prepared presentation and test sheet
16) finals week
17) finals week

Sources

Course Notes / Textbooks: Önceden hazırlamış sunum ve deney föyü
References: Pre-prepared presentation and test sheet

Course - Learning Outcome Relationship

No Effect 1 Lowest 2 Medium 3 Highest
       
Program Outcomes Level of Contribution
1) Having knowledge and understanding of Molecular Biology and Genetics subjects, established on competencies gained in previous education and supported by using course books containing latest information, application tools and other scientific literature. 2
2) Students can integrate knowledge and skills from molecular biology and genetics courses and can acquire further knowledge according to their own interests. 2
3) Students acquire practical skills in fundamental molecular biology and genetics techniques. 2
4) Ability of proposing solutions in unexpected, complicated situations on applications of Molecular Biology and Genetics by claiming responsibility individually or as a part of a team. 3
5) Competency in planning academic studies on Molecular Biology and Genetics and carrying out these studies individually or collectively. 3
6) Students can develop ability to analyse and interpret experimental data obtained in a laboratory setting statistically. 1
7) Sufficient foreign language knowledge for communication between colleagues and following literature on Molecular Biology and Genetics. 2
8) Students can use computational technologies to analyse scientific data and for information retrieval. 1
9) Being aware of the necessity of lifelong education, reaching information, following the advances in science and technology and constant struggle of renewing oneself. 1
10) Evaluating natural and social events with an environmental point of view and ability of informing and leading the public opinion 2
11) Students acquire professional knowledge and skills to fulfil requirements of their future employers. 3
12) Having proper social, ethical and scientific values and the will to protect these values on studies about collection, evaluation, contemplation, publication and application of data regarding Molecular Biology and Genetics. 1
13) Students can understand and evaluate advantages and limitations of technological platforms in life sciences including genomics, genetic engineering and biotechnology. 1
14) Students would have consciousness on subjects such as the quality management, worker welfare and safety. 2

Learning Activity and Teaching Methods

Labs

Measurement and Evaluation Methods and Criteria

Yazılı Sınav (Açık uçlu sorular, çoktan seçmeli, doğru yanlış, eşleştirme, boşluk doldurma, sıralama)
Uygulama
Raporlama

Assessment & Grading

Semester Requirements Number of Activities Level of Contribution
Application 14 % 40
Midterms 1 % 30
Final 1 % 30
total % 100
PERCENTAGE OF SEMESTER WORK % 70
PERCENTAGE OF FINAL WORK % 30
total % 100

İş Yükü ve AKTS Kredisi Hesaplaması

Activities Number of Activities Aktiviteye Hazırlık Aktivitede Harçanan Süre Aktivite Gereksinimi İçin Süre Workload
Course Hours 17 4 68
Study Hours Out of Class 14 1 14
Midterms 1 48 48
Final 1 48 48
Total Workload 178